ABSTRACT
Covid-19 is a severe acute respiratory syndrome, the disease presents with a ranging from asymptomatic to severe symptomatic illness with multiple organ failure and death, and can cause a severe effect on the coagulation system. This study aimed to determine the effect of the covid 19 on the extrinsic and intrinsic pathway of coagulation [prothrombin time(PT), international normalized ratio (INR), and activated partial thromboplastin time (APTT)] and to determine the association of age and gender with the severity of COVID-19 in Sudan in order to improve the outcome. A cross-sectional study carried out among 487 COVID-19 patients attending Khartoum State. COVID-19 patients were confirmed by RT-PCR. For all patients, the prothrombin times (PT), International normalized ratio (INR), and Activated partial thromboplastin (APTT) were estimated by using a semi-automated coagulometer analyzer. Patients were divided into three subclass groups according to the Severity of COVID-19 (mild, severe in the emergency room) (ER) and intensive care unit (ICU), and the clotting factors values were compared between the groups. The results were statically analyzed by spss version 21 for data analysis. These results showed statistically significant increased Levels of PT, INR, and APTT for all (P. value = 0.000), compared to the control group. Also, the levels of coagulation tests were higher in ICU COVID-19 patients (P. value = 0.000) compared to mild and severe subgroups. This study concluded that: coagulation clotting times were increased in COVID-19 patients, especially among patients in ICU which could be a marker for DIC and even death.
ABSTRACT
The electromagnetic field (EMF) of biomolecules can be utilized for detection and identification purposes. This paper is presenting a preliminary proof-of-concept experiment for rapid virus detection using an improved complementary split-ring resonator of mutually-coupled cells. Spectral responses of the sensor prototype have reliably indicated the presence of an Adeno-Associated Virus (AAV) in the hosting medium. Besides, two isolates of a fish virus were differentiated when tested in the near-field of the sensor. Promising results would revolutionize the space to develop an inexpensive, reusable, rapid EM testing technology for lab-on-chip virus detection to help combatting future pandemics. © 2022 IEEE.
ABSTRACT
SARS-CoV-2 is a systemic infection that has a significant impact on the hemostasis and hematopoietic system. Lymphopenia may be considered a cardinal laboratory finding, with prognostic potential. The study aimed to determine the differential leukocyte count in SARS-CoV-2 among Sudanese patients, during the period from March to December 2020. A total of 787 subjects were enrolled 487 patients with COVID-19 and 300 healthy volunteers as a control group;their ages ranged from 29 to 89 years. 3 ml of EDTA venous blood samples were collected from each participant standard for CBC investigation and then analyzed by SPSS version 21 (Mean and Standard deviation). A significant association between leukocyte count among ICU, ER group, and control with (P. value 0.000), in addition, a significant association was revealed among mild group and control group in differential neutrophil count, differential lymphocyte count, and absolute neutrophil count (p. value 0.000), however non-significant in TWBC absolute mixed cell count, absolute lymphocyte count, and differential mixed cells count (value 0.7,0.2, 0.19) respectively. The study concluded that leukocytosis with neutrophilia and lymphopenia is associated with the severity of SARS-CoV-2 infection, and should be implicated as predict for a serious course of the disease as well as mortality.
ABSTRACT
The relevance of coagulation malfunction in COVID-19 (severe coronavirus disease) is ambiguous. Current study aimed to assess the coagulation among SARS-CoV-2 hospitalized patients. A cross sectional study with qualitative approach was conducted among 300 patients who are already diagnosed as COVID 19 compared to 300 apparently healthy control group attended to Red Sea State during study period from April 2020 to April 2021. The Humaclot Due Plus1 coagulation analyser was used to estimate the prothrombin time (PT), activated partial prothrombin time (APTT), and international normalized ratio (INR) (Wiesbaden 1, Germany), adding 25 μL of plasma in cuvette. The study result showed that in COVID-19 patients D.dimer level is high (2000-10000 ng/mL) compared with control group (up to 500 ng/mL). COVID-19 infection cause high D. dimer level which can lead to thrombosis event or bleeding tendency. Abnormal coagulation results were revealed among SARS-CoV-2, with markedly elevated D. dimer.